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Negative data values appear in the space from x 0 to x w, and positive data values are plotted between x w and x m (where the top data value T occurs). The display is defined for x in the range from 0 to m. Therefore, plot scaling is not simply cosmetic, it affects the algorithm performance. For a 4.5 decade, display range m 4.5 ln(10) 10.36.
#Biexponential transformation flowjo 10 software#
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5 or above is required for correct Logicle and Hyperlog transformations. Go to Platform Biexponential Transformation Manually specify Transform 2. Version 0.6 added experimental support for exporting FlowJo 10 workspace files. CytoFLEX and CytExpert are trademarks of Xitogen Technologies (Suzhou), Inc., a Beckman Coulter company. FlowJo v9.7.7 When transporting CytExpert data files into FlowJo v9.7.7 the data appears as below: IC6.fcs compensated FL7-A CD3 APC-A700-A A-10 3-10 2 10 3 10 4 10 5 10 6-10 3-10 2 10 3 10 4 10 5 10 6. Beckman Coulter, the stylized logo, Kaluza are trademarks of Beckman Coulter, Inc. 20 FlowJo v8.3.3 (Rm 120 G5): BiExponential Transformation of Specimen 1 Tube 1. Unfortunately, for this version (9.7.7), FlowJo does not allow the user to set the maximum value to be viewed therefore, the decade display cannot be changed and stayed as a 7-decade plot.įor Research Use Only. 10 Compensation: False Positive CD4 Response to CEF Pepmix mSA EOLm. In the “Edit Biexponential Transformation Settings” window, enter values that are higher than the default value then press enter, followed by selecting “Apply Now”. Go to Platform – Biexponential Transformation – Manually specify TransformĢ. This view is now similar to the data as seen in the CytoFLEX CytExpert plot.ġ. If the default settings are used in FlowJo, when digital data (fcs3. When transporting CytExpert data files into FlowJo V9.7.7 the data appears as here: The Herzenberg paper (review of logicle biexponential transformation) gives an excellent explanation of the transform and data display in general and we encourage anyone interested in the details of biexponential logicle transformation to read it FlowJo and Logicle. The new transformation appears in the plot: